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Stomatologija 2017; 19 (3):71-7 276 KB

Signalling molecules in jaw bones and gingival tissues of patients with Class II and Class III dentofacial deformities

Iveta Jankovska¹, Mara Pilmane², Ilga Urtane¹

Summary

Objectives. To detect signalling molecule specificities in jaw bone growth zones in skeletal class II and class III patients and compare them to those of a control group.

Material and Methods. Twenty skeletal Class II and 20 skeletal Class III patients who underwent orthognathic surgery treatment were in the study group and five skeletal Class I patients who had impacted third molars extracted were in the control group. During the orthognathic surgery, tissue samples were taken from the tuber maxillae, ramus mandibulae anterior and posterior part together with mucosa from the gingival transitory fold in the second molar region of the lower jaw. The samples were stained to detect TGF-β, BMP2/4, FGFR1, VEGF, OC, OP and MMP2 expression. The distributions of these factors were assessed semiquantitatively.

Results. We observed significant expression of TGF-β, BMP2/4, OC and OP in the bone tissue of the study group. FGFR1 expression was more pronounced only in mucosa. VEGF and MMP2 were found only in some tissue samples. More apoptotic cells were observed in the bone tissue and soft tissue of the control patients than in those of the skeletal Class II and Class III patients, in which apoptotic cell frequencies were relatively equal.

Conclusion. From bone tissue in tuber maxillae region the greater TGF-β and BMP2/4 expression is seen in Class III and control groups, comparing to Class II. In ramus mandibulae anterior part the expression of significant factors in bone tissue growth (TGF-β un BMP2/4) is higher in the control group and Class II patients, while in ramus mandibulae posterior part higher expression of TGF-β and BMP2/4 is in Class III patients, comparing to Class II, which indicates to a preserved growth potential in these jaw bone regions. More active bone extracellular matrix protein (osteocalcin and osteopontin) expression in tuber maxillae region both in class II and class III patient groups and different expression in ramus mandibulae anterior part, prove to the bone mineralization and metabolism activity changes, which, perhaps, characterize just these dentofacial deformations.

Key words: orthognathic surgery, bone, signalling molecules, immunohistochemistry

Received: 14 05 2014

Accepted for publishing: 28 09 2017

¹Department of Orthodontics, Institute of Stomatology, Riga Stradins University, Riga, Latvia

²Department of Morphology, Institute of Anatomy and Anthropology, Riga Stradins University, Riga, Latvia

Address correspondence to Dr. Iveta Jankovska, Department of Orthodontics, Institute of Stomatology, Riga Stradins University, 20 Dzirciema Street, Riga, Latvia.

E-mail address: iveta.j@hotmail.com



	Baltic Dental and Maxillofacial Journal

September, 2017, Vol. 19, No. 3 CONTENTS SCIENTIFIC ARTICLES Signalling molecules in jaw bones and gingival tissues of patients with Class II and Class III dentofacial deformities Iveta Jankovska, Mara Pilmane, Ilga Urtane 71-77 Family history and risk factors for cleft lip and palate patients and their associated anomalies Abdolreza Jamilian, Farzin Sarkarat, Mehrdad Jafari, Morteza Neshandar, Ehsan Amini, Saeed Khosravi, Alireza Ghassemi 78-83 Psychosocial factors correlated with children’s dental anxiety Līga Kroniņa, Malgožata Rasčevska, Rūta Care 84-90 Serum and salivary lactate dehydrogenase levels as biomarkers of tissue damage among cigarette smokers. A bochemical study Kumuda Rao, Subhas Babu, Urvashi A. Shetty, Renita Lorina Castelino, Shishir Ram Shetty 91-96 CASE REPORT Histopathological and microradiological features of peri-implantits. A case report Mustafa Ayna, Aydin Gulses, Yahya Açili 97-100 © 2017 Stomatologija		Stomatologija 2017; 19 (3):71-7 276 KB Signalling molecules in jaw bones and gingival tissues of patients with Class II and Class III dentofacial deformities Iveta Jankovska¹, Mara Pilmane², Ilga Urtane¹ Summary Objectives. To detect signalling molecule specificities in jaw bone growth zones in skeletal class II and class III patients and compare them to those of a control group. Material and Methods. Twenty skeletal Class II and 20 skeletal Class III patients who underwent orthognathic surgery treatment were in the study group and five skeletal Class I patients who had impacted third molars extracted were in the control group. During the orthognathic surgery, tissue samples were taken from the tuber maxillae, ramus mandibulae anterior and posterior part together with mucosa from the gingival transitory fold in the second molar region of the lower jaw. The samples were stained to detect TGF-β, BMP2/4, FGFR1, VEGF, OC, OP and MMP2 expression. The distributions of these factors were assessed semiquantitatively. Results. We observed significant expression of TGF-β, BMP2/4, OC and OP in the bone tissue of the study group. FGFR1 expression was more pronounced only in mucosa. VEGF and MMP2 were found only in some tissue samples. More apoptotic cells were observed in the bone tissue and soft tissue of the control patients than in those of the skeletal Class II and Class III patients, in which apoptotic cell frequencies were relatively equal. Conclusion. From bone tissue in tuber maxillae region the greater TGF-β and BMP2/4 expression is seen in Class III and control groups, comparing to Class II. In ramus mandibulae anterior part the expression of significant factors in bone tissue growth (TGF-β un BMP2/4) is higher in the control group and Class II patients, while in ramus mandibulae posterior part higher expression of TGF-β and BMP2/4 is in Class III patients, comparing to Class II, which indicates to a preserved growth potential in these jaw bone regions. More active bone extracellular matrix protein (osteocalcin and osteopontin) expression in tuber maxillae region both in class II and class III patient groups and different expression in ramus mandibulae anterior part, prove to the bone mineralization and metabolism activity changes, which, perhaps, characterize just these dentofacial deformations. Key words: orthognathic surgery, bone, signalling molecules, immunohistochemistry Received: 14 05 2014 Accepted for publishing: 28 09 2017 ¹Department of Orthodontics, Institute of Stomatology, Riga Stradins University, Riga, Latvia ²Department of Morphology, Institute of Anatomy and Anthropology, Riga Stradins University, Riga, Latvia Address correspondence to Dr. Iveta Jankovska, Department of Orthodontics, Institute of Stomatology, Riga Stradins University, 20 Dzirciema Street, Riga, Latvia. E-mail address: iveta.j@hotmail.com