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Stomatologija 2020; 22 (1): 3-8 186 KB

Tendencies of FGFR2 rs2981582 polymorphism in patients with oral cancer

Vykintas Liutkevicius¹, Toma Tamauskaite², Alvita Vilkeviciute³, Rasa Liutkeviciene³, Rasa Smalinskaite², Agne Giedraitiene⁴, Alina Smalinskiene⁵, Virgilijus Uloza¹

Summary

Objective. Fibroblast growth factor receptor 2 (FGFR2) is a member of the FGFR family of tyrosine kinase receptors, which via cell growth, invasiveness, motility and angiogenesis contributes to the process of tumorogenesis. A huge interest today is focused on FGFR2 gene polymorphism as it may have a significant impact on the development of various benign and malignant tumors.

A case-control study was designed to help determine if FGFR2 gene polymorphism rs2981582 is associated with oral cancer in Lithuanian subjects.

Methods. The study included 35 patients with a diagnosis of oral cancer and 100 healthy subjects as a reference group. DNA samples were extracted from peripheral venous blood. Genotyping of FGFR2 rs2981582 was performed using the real-time polymerase chain reaction method. Statistical analysis was performed using „IBM SPSS Statistics 20.0“.

Results. It was determined that FGFR2 gene rs2981582 polymorphism has no effect on a development of oral cancer. The analysis of FGFR2 gene polymorphisms did not reveal any differences in the distribution of GG, GA, and AA genotypes between the oral cancer group, and the control group (42.9%, 48.6%, and 8.6% vs. 46%, 37% and 17%, respectively).

Conclusion. Results of present study showed no association between FGFR2 gene polymorphisms rs2981582 and oral cancer. However, a further study with a larger sample sizes is advisable.

Key words: fibroblast growth factor receptor 2, gene polymorphism, oral cancer.

Received: 18 02 2019

Accepted for publishing: 21 03 2020

¹Department of Otolaryngology, Academy of Medicine, Lithuanian University of Health Sciences, Kaunas, Lithuania

²Academy of Medicine, Lithuanian University of Health Sciences, Kaunas, Lithuania

³Neuroscience Institute, Lithuanian University of Health Sciences, Kaunas, Lithuania

⁴Institute of Microbiology and Virology, Lithuanian University of Health Sciences, Kaunas, Lithuania

⁵Institute of Cardiology, Medicine Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania

Address correspondence to Rasa Liutkeviciene, Neuroscience Institute, Lithuanian University of Health Sciences, Kaunas, Lithuania, Eivenių 2, Kaunas, Lithuania.

E-mail address: rliutkeviciene@gmail.com



	Baltic Dental and Maxillofacial Journal

March, 2020, Vol. 22, No. 1 CONTENTS SCIENTIFIC ARTICLES Tendencies of FGFR2 rs2981582 polymorphism in patients with oral cancer Vykintas Liutkevicius, Toma Tamauskaite, Alvita Vilkeviciute, Rasa Liutkeviciene, Rasa Smalinskaite, Agne Giedraitiene, Alina Smalinskiene, Virgilijus Uloza 3-8 REVIEWS Basal Cell Carcinoma. Analysis of 395 cases localized in the neck, ear and nose region Egils Kornevs, Ingus Arnolds Apse, Toms Janis Safronovs, Aija Krastina, Arturs Paparde, Gunars Lauskis, Girts Salms 9-16 Sodium hypochlorite accidents in dentistry. A systematic review of published case reports Shishir Ram Shetty, Sura Ali Ahmed Fuoad Al-Bayati, Ashwin Narayanan, Mohammed Said Hamed, Hossam Abdelatty Eid Abdemagyd, Praveen Shetty 17-22 Autologous platelet concentrates in treatment of medication related osteonecrosis of the jaw Henrikas Rusilas, Aušra Balčiūnaitė, Juozas Žilinskas 23-27 CASE REPORT Clear cell odontogenic carcinoma: a rare pathology with an innovative resolution Natalia Lucía Gómez, Luis Boccalatte, Alejandro Yanzón, María Gabriela Nassif, Juan José Larrañaga, Marcelo Fernando Figari 28-32 © 2020 Stomatologija		Stomatologija 2020; 22 (1): 3-8 186 KB Tendencies of FGFR2 rs2981582 polymorphism in patients with oral cancer Vykintas Liutkevicius¹, Toma Tamauskaite², Alvita Vilkeviciute³, Rasa Liutkeviciene³, Rasa Smalinskaite², Agne Giedraitiene⁴, Alina Smalinskiene⁵, Virgilijus Uloza¹ Summary Objective. Fibroblast growth factor receptor 2 (FGFR2) is a member of the FGFR family of tyrosine kinase receptors, which via cell growth, invasiveness, motility and angiogenesis contributes to the process of tumorogenesis. A huge interest today is focused on FGFR2 gene polymorphism as it may have a significant impact on the development of various benign and malignant tumors. A case-control study was designed to help determine if FGFR2 gene polymorphism rs2981582 is associated with oral cancer in Lithuanian subjects. Methods. The study included 35 patients with a diagnosis of oral cancer and 100 healthy subjects as a reference group. DNA samples were extracted from peripheral venous blood. Genotyping of FGFR2 rs2981582 was performed using the real-time polymerase chain reaction method. Statistical analysis was performed using „IBM SPSS Statistics 20.0“. Results. It was determined that FGFR2 gene rs2981582 polymorphism has no effect on a development of oral cancer. The analysis of FGFR2 gene polymorphisms did not reveal any differences in the distribution of GG, GA, and AA genotypes between the oral cancer group, and the control group (42.9%, 48.6%, and 8.6% vs. 46%, 37% and 17%, respectively). Conclusion. Results of present study showed no association between FGFR2 gene polymorphisms rs2981582 and oral cancer. However, a further study with a larger sample sizes is advisable. Key words: fibroblast growth factor receptor 2, gene polymorphism, oral cancer. Received: 18 02 2019 Accepted for publishing: 21 03 2020 ¹Department of Otolaryngology, Academy of Medicine, Lithuanian University of Health Sciences, Kaunas, Lithuania ²Academy of Medicine, Lithuanian University of Health Sciences, Kaunas, Lithuania ³Neuroscience Institute, Lithuanian University of Health Sciences, Kaunas, Lithuania ⁴Institute of Microbiology and Virology, Lithuanian University of Health Sciences, Kaunas, Lithuania ⁵Institute of Cardiology, Medicine Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania Address correspondence to Rasa Liutkeviciene, Neuroscience Institute, Lithuanian University of Health Sciences, Kaunas, Lithuania, Eivenių 2, Kaunas, Lithuania. E-mail address: rliutkeviciene@gmail.com