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Summary

Alterations of neutrophils function by tobacco products may play a central role in the pathogenesis of periodontal diseases and several smoking related systemic diseases. The aim of the study was to assess a response of peripheral blood neutrophils unstimulated and stimulated by prodigiosan to smoking.

Materials and methods. The study included 17 smoking men that addressed for treatment of various odontological problems to out patient department of Kaunas University Clinic. The subjects were 22-43 years of age and systemic diseases free. All subjects answered to questions about smoking habits. To assess the response of neutrofils (unstimulated and stimulated by prodigiosan) to smoking the chemiliuminescence and nitroblue tetrazolium test were used to measure their oxidative metabolism.

Results. After smoking both neutrophils unstimulated and stimulated with prodigiosan showed considerable extracellular chemiliuminescence responses, but the first one reached maximal value in 45 min, while the second one in 15 min after smoking. There was no total (intra and extracellular) chemiliuminescence response of neutrophils unstimulated and stimulated by prodigiosan to smoking. Neutrofils exposed to smoking showed a significant increase in their nitroblue tetrazolium reduction.

Conclusion. Both stimulated by prodigiosan and unstimulated peripheral blood neutrophils release reactive oxygen species extracellulary which may alter the pathogenic processes in periodontal and other systemic diseases.

Key words: neutrophils, smoking, prodigiosan, reactive oxygen species, chemiluminescence, nitroblue tetrazolium reduction test.

Received: 20 04 2005

Accepted for publishing: 20 06 2005

Darius Zasimauskas - D.D.S., assist. prof., Clinic of Prosthetic Denstistry, Kaunas Medical University, Lithuania.

Gediminas Zekonis - D.D.S., PhD, assoc. prof., Clinic of Prosthetic Denstistry, Kaunas Medical University, Lithuania.

Address correspondence to Gediminas Zekonis, Clinic of Prosthetic Dentistry, Hospital of Kaunas University of Medicine, Sukilelių 51, Kaunas, Lithuania.
E-mail: zekonisg@hotmail.com



	Baltic Dental and Maxillofacial Journal

June, 2005, Vol. 7, No. 2 CONTENTS SCIENTIFIC ARTICLES Comparative analysis of the effectiveness of the mandibular angle fracture treatment methods 35 - 39 Chemomechanical caries removal for children 40 - 44 The microbiological status of patients with periodontitis in Southern Estonia after non-surgical periodontal therapy 45 - 47 The response of peripheral blood neutrophils unstimulated and stimulated by prodigiosan to smoking 48 - 51 Effectiveness evaluation of different suction systems 52 - 57 Dietary and oral hygiene habits in children with type I Diabetes Mellitus related to dental caries 58 - 62 © 2005 Stomatologija		Stomatologija 2005; 7 (2): 48-51 190 KB The Response of Peripheral Blood Neutrophils Unstimulated and Stimulated by Prodigiosan to Smoking Darius Zasimauskas, Gediminas Žekonis Summary Alterations of neutrophils function by tobacco products may play a central role in the pathogenesis of periodontal diseases and several smoking related systemic diseases. The aim of the study was to assess a response of peripheral blood neutrophils unstimulated and stimulated by prodigiosan to smoking. Materials and methods. The study included 17 smoking men that addressed for treatment of various odontological problems to out patient department of Kaunas University Clinic. The subjects were 22-43 years of age and systemic diseases free. All subjects answered to questions about smoking habits. To assess the response of neutrofils (unstimulated and stimulated by prodigiosan) to smoking the chemiliuminescence and nitroblue tetrazolium test were used to measure their oxidative metabolism. Results. After smoking both neutrophils unstimulated and stimulated with prodigiosan showed considerable extracellular chemiliuminescence responses, but the first one reached maximal value in 45 min, while the second one in 15 min after smoking. There was no total (intra and extracellular) chemiliuminescence response of neutrophils unstimulated and stimulated by prodigiosan to smoking. Neutrofils exposed to smoking showed a significant increase in their nitroblue tetrazolium reduction. Conclusion. Both stimulated by prodigiosan and unstimulated peripheral blood neutrophils release reactive oxygen species extracellulary which may alter the pathogenic processes in periodontal and other systemic diseases. Key words: neutrophils, smoking, prodigiosan, reactive oxygen species, chemiluminescence, nitroblue tetrazolium reduction test. Received: 20 04 2005 Accepted for publishing: 20 06 2005 Darius Zasimauskas - D.D.S., assist. prof., Clinic of Prosthetic Denstistry, Kaunas Medical University, Lithuania. Gediminas Zekonis - D.D.S., PhD, assoc. prof., Clinic of Prosthetic Denstistry, Kaunas Medical University, Lithuania. Address correspondence to Gediminas Zekonis, Clinic of Prosthetic Dentistry, Hospital of Kaunas University of Medicine, Sukilelių 51, Kaunas, Lithuania. E-mail: zekonisg@hotmail.com