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Summary

Our objective was to investigate the presence of periodontal pathogens in comparison with the total degree of microorganisms after non-surgical periodontal therapy. The study material consisted of microbiological samples from periodontal pockets originating from 140 consecutive patients with chronic generalized severe periodontitis. The subgingival samples from periodontal pockets were obtained by a sterile curette, placed into 2 ml of the VMGA III medium, homogenized and serially diluted in the Brucella broth. 100 µl aliquots from the dilutions were inoculated onto the Brucella and the TSBV agar. The plates were incubated in an anaerobic chamber and under microaerobic conditions. The isolates were identified according to colonial and cellular morphology, the potency disk pattern, and the biochemical profiles. After instrumentation, no periodontal pathogens were isolated in 46 (33%) patients, while 94 patients (67%) were infected with one to five different periodontal pathogens. However, higher degree of the total microflora was positively correlated with number of isolated pathogens, a putative indicator of their presence. Therefore, due to the occurrence of residual microorganisms after non-surgical mechanical treatment, information about the pattern of these pathogens is needed for application of antimicrobial therapy. The level of microbial load in gingival pockets, including both pathogenic and non-pathogenic species, is one of the determinants of presence of residual pathogens after non-surgical periodontal therapy.

Key words: invasive pathogens antimicrobial susceptibility.

Received: 15 04 2005

Accepted for publishing: 20 06 2005

¹Department of Clinical Microbiology, United Laboratories of Tartu University Clinics.

²University of Tartu, Medical Faculty, Department of Stomatology.

Krista Lõivukene¹ - MD, PhD, clinical microbiologist.

Paul Naaber¹ - MD, PhD, Head of Department of Microbiology of United Laboratories of Tartu.

Mare Saag² - MD, PhD, professor; Head of the Department of Stomatology.

Ene-Renate Pähkla² - MD, dentist, lecturer.

Taive Koppel² - MD, dentist, lecturer.

Address correspondence to Krista Lõivukene, Department of Clinical Microbiology, United Laboratories of Tartu University Clinics, Puusepa 1A, Tartu 50406, Estonia.

E-mail: krista.loivuke@kliinikum.ee



	Baltic Dental and Maxillofacial Journal

June, 2005, Vol. 7, No. 2 CONTENTS SCIENTIFIC ARTICLES Comparative analysis of the effectiveness of the mandibular angle fracture treatment methods 35 - 39 Chemomechanical caries removal for children 40 - 44 The microbiological status of patients with periodontitis in Southern Estonia after non-surgical periodontal therapy 45 - 47 The response of peripheral blood neutrophils unstimulated and stimulated by prodigiosan to smoking 48 - 51 Effectiveness evaluation of different suction systems 52 - 57 Dietary and oral hygiene habits in children with type I Diabetes Mellitus related to dental caries 58 - 62 © 2005 Stomatologija		Stomatologija 2005; 7 (2): 45-47 194 KB The Microbiological Status of Patients with Periodontitis in Southern Estonia after Non-surgical Periodontal Therapy Krista Lõivukene, Ene-Renate Pähkla, Taive Koppel, Mare Saag, Paul Naaber Summary Our objective was to investigate the presence of periodontal pathogens in comparison with the total degree of microorganisms after non-surgical periodontal therapy. The study material consisted of microbiological samples from periodontal pockets originating from 140 consecutive patients with chronic generalized severe periodontitis. The subgingival samples from periodontal pockets were obtained by a sterile curette, placed into 2 ml of the VMGA III medium, homogenized and serially diluted in the Brucella broth. 100 µl aliquots from the dilutions were inoculated onto the Brucella and the TSBV agar. The plates were incubated in an anaerobic chamber and under microaerobic conditions. The isolates were identified according to colonial and cellular morphology, the potency disk pattern, and the biochemical profiles. After instrumentation, no periodontal pathogens were isolated in 46 (33%) patients, while 94 patients (67%) were infected with one to five different periodontal pathogens. However, higher degree of the total microflora was positively correlated with number of isolated pathogens, a putative indicator of their presence. Therefore, due to the occurrence of residual microorganisms after non-surgical mechanical treatment, information about the pattern of these pathogens is needed for application of antimicrobial therapy. The level of microbial load in gingival pockets, including both pathogenic and non-pathogenic species, is one of the determinants of presence of residual pathogens after non-surgical periodontal therapy. Key words: invasive pathogens antimicrobial susceptibility. Received: 15 04 2005 Accepted for publishing: 20 06 2005 ¹Department of Clinical Microbiology, United Laboratories of Tartu University Clinics. ²University of Tartu, Medical Faculty, Department of Stomatology. Krista Lõivukene¹ - MD, PhD, clinical microbiologist. Paul Naaber¹ - MD, PhD, Head of Department of Microbiology of United Laboratories of Tartu. Mare Saag² - MD, PhD, professor; Head of the Department of Stomatology. Ene-Renate Pähkla² - MD, dentist, lecturer. Taive Koppel² - MD, dentist, lecturer. Address correspondence to Krista Lõivukene, Department of Clinical Microbiology, United Laboratories of Tartu University Clinics, Puusepa 1A, Tartu 50406, Estonia. E-mail: krista.loivuke@kliinikum.ee