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Stomatologija 2016; 18 (1): 9-13 528 KB

Hypericum perforatum L. treatment restored bone mass changes in swimming stressed rats

Nikos Seferos, Loukas Petrokokkinos, Antonia Kotsiou, George Rallis, Christine Tesseromatis

Summary

Stress, via corticosteroids release, influences bone mass density. Hypericum perforatum (Hp) a traditional remedy possess antidepressive activity (serotonin reuptake inhibitor) and wound healing properties. Hp preparation contains mainly hypericin, hyperforin, hyperoside and flavonoids exerting oestrogen-mimetic effect. Cold swimming represents an experimental model of stress associating mental strain and corporal exhaustion.

Objectives. This study investigates the Hp effect on femur and mandible bone mass changes in rats under cold forced swimming procedure.

Methods. 30 male Wistar rats were randomized into three groups. Group A was treated with Methanolic extract of Hp (Jarsin®) via gastroesophageal catheter, and was submitted to cold swimming stress for 10 min/daily. Group B was submitted to cold stress, since group C served as control. Experiment duration was 10 days. Haematocrite and serum free fatty acids (FFA) were estimated. Furthermore volume and specific weight of each bone as well as bone mass density via dual energy X-Ray absorptiometry (DEXA) were measured. Statistic analysis by t-test. Results. Hp treatment restores the stress injuries. Adrenals and bone mass density regain their normal values.

Conclusions. Injuries occurring by forced swimming stress in the rats are significantly improved by Hp treatment. Estrogen-like effects of Hp flavonoids eventually may act favorable in bone remodeling.

Key words: swimming stress, bone mass, Hypericum perforatum, rats.

Received: 03 03 2014

Accepted for publishing: 28 03 2016

¹Department of Pharmacology, Medical School, University of Athens, Greece

²Department of Medical Physics Lab, Medical School, University of Athens, Greece

³Aretaieion University Hospital, Athens, Greece

⁴Department of Oral and Maxillofacial Surgery, General Hospital of Athens "KAT", Greece

Nikos Seferos¹ – dental doctor

Loukas Petrokokkinos² – physicist, MSc

Antonia Kotsiou³ – pharmacist, PhD, lecturer

George Rallis⁴ – Dr. Med, Dr. Dent, PhD Christine Tesseromatis1 – MD, MDD, assoc. prof

Address correspondence to Dr. Christine Tesseromatis, Department of Pharmacology, Medical School, University of Athens, Mikras Asias 75, Goudi 11527, Greece.

E-mail address: ctesser@med.uoa.gr



	Baltic Dental and Maxillofacial Journal

March, 2016, Vol. 18, No. 1 CONTENTS REVIEW Prevention and treatment of white spot lesions during and after fixed orthodontic treatment: A systematic literature review Egle Lapenaite, Kristina Lopatiene, Aira Ragauskaite 3-8 SCIENTIFIC ARTICLES Hypericum perforatum L. treatment restored bone mass changes in swimming stressed rats Nikos Seferos, Loukas Petrokokkinos, Antonia Kotsiou, George Rallis, Christine Tesseromatis 9-13 Factors influencing the caries experience of 6 and 12 year old children in Riga, Latvia Jekaterina Gudkina, Anda Brinkmane, Stephen H. Abrams, Bennett T. Amaechi 14-20 Vertical and sagittal morphology of the facial skeleton and the pharyngeal airway Kristina Lopatienė, Agnė Dabkutė, Viktorija Juškevičiūtė 21-25 Prevention of Occlusal Caries using a Ozone, Sealant and Fluoride Varnish in Children Julija Kalnina, Ruta Care 26-31 CASE REPORT Direct composite resin crown fabrication on a custom formed root canal post – EverStick®POST Valdas Vilkinis, Juozas Žilinskas 32-36 © 2016 Stomatologija		Stomatologija 2016; 18 (1): 9-13 528 KB Hypericum perforatum L. treatment restored bone mass changes in swimming stressed rats Nikos Seferos, Loukas Petrokokkinos, Antonia Kotsiou, George Rallis, Christine Tesseromatis Summary Stress, via corticosteroids release, influences bone mass density. Hypericum perforatum (Hp) a traditional remedy possess antidepressive activity (serotonin reuptake inhibitor) and wound healing properties. Hp preparation contains mainly hypericin, hyperforin, hyperoside and flavonoids exerting oestrogen-mimetic effect. Cold swimming represents an experimental model of stress associating mental strain and corporal exhaustion. Objectives. This study investigates the Hp effect on femur and mandible bone mass changes in rats under cold forced swimming procedure. Methods. 30 male Wistar rats were randomized into three groups. Group A was treated with Methanolic extract of Hp (Jarsin®) via gastroesophageal catheter, and was submitted to cold swimming stress for 10 min/daily. Group B was submitted to cold stress, since group C served as control. Experiment duration was 10 days. Haematocrite and serum free fatty acids (FFA) were estimated. Furthermore volume and specific weight of each bone as well as bone mass density via dual energy X-Ray absorptiometry (DEXA) were measured. Statistic analysis by t-test. Results. Hp treatment restores the stress injuries. Adrenals and bone mass density regain their normal values. Conclusions. Injuries occurring by forced swimming stress in the rats are significantly improved by Hp treatment. Estrogen-like effects of Hp flavonoids eventually may act favorable in bone remodeling. Key words: swimming stress, bone mass, Hypericum perforatum, rats. Received: 03 03 2014 Accepted for publishing: 28 03 2016 ¹Department of Pharmacology, Medical School, University of Athens, Greece ²Department of Medical Physics Lab, Medical School, University of Athens, Greece ³Aretaieion University Hospital, Athens, Greece ⁴Department of Oral and Maxillofacial Surgery, General Hospital of Athens "KAT", Greece Nikos Seferos¹ – dental doctor Loukas Petrokokkinos² – physicist, MSc Antonia Kotsiou³ – pharmacist, PhD, lecturer George Rallis⁴ – Dr. Med, Dr. Dent, PhD Christine Tesseromatis1 – MD, MDD, assoc. prof Address correspondence to Dr. Christine Tesseromatis, Department of Pharmacology, Medical School, University of Athens, Mikras Asias 75, Goudi 11527, Greece. E-mail address: ctesser@med.uoa.gr