|Baltic Dental and Maxillofacial Journal|
December, 2013, Vol. 15, No. 4
Influence of restorative techniques on fracture load of endodontically treated premolars
© 2014 Stomatologija
Stomatologija 2013; 15 (4): 129-134 455 KB
Apoptosis and MMP-2, TIMP-2 expression in cleft lip and palate
Liene Smane, Mara Pilmane, Ilze Akota
Aim of our study was complex detection of appearance and distribution of specific signalling proteins and apoptosis in facial tissue of children with complete bilateral cleft lip and palate (CBCLP).
Materials and methods. Nineteen CBCLP patients and 11 unaffected subjects were involved in this study. All the tissue samples were proceeded for detection of matrix metalloproteinase-2 (MMP-2), tissue inhibitor of matrix metalloproteinase-2 (TIMP-2), and apoptosis. The intensity of immunostaining was graded semi-quantitatively.
Results of the terminal deoxynucleotidyl transferase mediated dUTP nick-end labeling (TUNEL) method were obtained by counting apoptosis positive cells in five unintentionally chosen fields of vision. Groups were compared using the Mann-Whitney test. Results. TUNEL-positive oral epithelial cells were significantly increased in the control group when compared with the CBCLP group. Connective tissue cells have a statistically significant lower expression of TIMP-2 in the control group compared to the CBCLP group.
Conclusions. TIMP-2 positive connective tissue cells increasingly found in oral mucosa lamina propria proves the decrease of local apoptosis in CLP patients. Prominent expression of MMP-2 in cleft affected soft tissue indicates a possible increase of tissue remodelling.
Key words: signalling proteins, apoptosis, cleft lip and palate, children.
Received: 19 06 2013
Accepted for publishing: 23 12 2013
1Department of Morphology, Institute of Anatomy and Anthropology, Riga Stradins University, Latvia
2Institute of Stomatology, Riga Stradins University, Latvia
Liene Smane1 – PhD student, lecturer
Mara Pilmane1 – PhD., Dr. habil. med, prof.
Ilze Akota2 – D.D.S., MSc (Oslo), PhD., prof.
Address correspondence to Liene Smane, Dzirciema street 16, Riga, LV-1007, Latvia.
E-mail address: Liene.Smane@rsu.lv