Baltic Dental and Maxillofacial Journal
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December, 2013, Vol. 15, No. 4



Expression of growth factors and growth factor receptors in human cleft-affected tissue
Benita Krivicka, Mara Pilmane, Ilze Akota

Radiographic assessment of findings in the maxillary sinus using cone-beam computed tomography
Ilze Dobele, Ligija Kise, Peteris Apse, Gints Kragis, Andris Bigestans

Influence of restorative techniques on fracture load of endodontically treated premolars
Ana Amélia Bianchi e Silva, Paula Cristine Ghiggi, Eduardo Gonçalves Mota, Gilberto Antonio Borges, Luiz Henrique Burnett Júnior, Ana Maria Spohr

Apoptosis and MMP-2, TIMP-2 expression in cleft lip and palate
Liene Smane, Mara Pilmane, Ilze Akota

Toothache among dental patients attending a Nigerian secondary healthcare setting
Clement Chinedu Azodo, Oritseweyemi Ololo

© 2014 Stomatologija

Stomatologija 2013; 15 (4): 129-134 455 KB

Apoptosis and MMP-2, TIMP-2 expression in cleft lip and palate

Liene Smane, Mara Pilmane, Ilze Akota


Aim of our study was complex detection of appearance and distribution of specific signalling proteins and apoptosis in facial tissue of children with complete bilateral cleft lip and palate (CBCLP).

Materials and methods. Nineteen CBCLP patients and 11 unaffected subjects were involved in this study. All the tissue samples were proceeded for detection of matrix metalloproteinase-2 (MMP-2), tissue inhibitor of matrix metalloproteinase-2 (TIMP-2), and apoptosis. The intensity of immunostaining was graded semi-quantitatively.

Results of the terminal deoxynucleotidyl transferase mediated dUTP nick-end labeling (TUNEL) method were obtained by counting apoptosis positive cells in five unintentionally chosen fields of vision. Groups were compared using the Mann-Whitney test. Results. TUNEL-positive oral epithelial cells were significantly increased in the control group when compared with the CBCLP group. Connective tissue cells have a statistically significant lower expression of TIMP-2 in the control group compared to the CBCLP group.

Conclusions. TIMP-2 positive connective tissue cells increasingly found in oral mucosa lamina propria proves the decrease of local apoptosis in CLP patients. Prominent expression of MMP-2 in cleft affected soft tissue indicates a possible increase of tissue remodelling.

Key words: signalling proteins, apoptosis, cleft lip and palate, children.

Received: 19 06 2013

Accepted for publishing: 23 12 2013

1Department of Morphology, Institute of Anatomy and Anthropology, Riga Stradins University, Latvia

2Institute of Stomatology, Riga Stradins University, Latvia

Liene Smane1 – PhD student, lecturer

Mara Pilmane1 – PhD., Dr. habil. med, prof.

Ilze Akota2 – D.D.S., MSc (Oslo), PhD., prof.

Address correspondence to Liene Smane, Dzirciema street 16, Riga, LV-1007, Latvia.

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