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Stomatologija 2013; 15 (4): 129-134 455 KB

Apoptosis and MMP-2, TIMP-2 expression in cleft lip and palate

Liene Smane, Mara Pilmane, Ilze Akota

Summary

Aim of our study was complex detection of appearance and distribution of specific signalling proteins and apoptosis in facial tissue of children with complete bilateral cleft lip and palate (CBCLP).

Materials and methods. Nineteen CBCLP patients and 11 unaffected subjects were involved in this study. All the tissue samples were proceeded for detection of matrix metalloproteinase-2 (MMP-2), tissue inhibitor of matrix metalloproteinase-2 (TIMP-2), and apoptosis. The intensity of immunostaining was graded semi-quantitatively.

Results of the terminal deoxynucleotidyl transferase mediated dUTP nick-end labeling (TUNEL) method were obtained by counting apoptosis positive cells in five unintentionally chosen fields of vision. Groups were compared using the Mann-Whitney test. Results. TUNEL-positive oral epithelial cells were significantly increased in the control group when compared with the CBCLP group. Connective tissue cells have a statistically significant lower expression of TIMP-2 in the control group compared to the CBCLP group.

Conclusions. TIMP-2 positive connective tissue cells increasingly found in oral mucosa lamina propria proves the decrease of local apoptosis in CLP patients. Prominent expression of MMP-2 in cleft affected soft tissue indicates a possible increase of tissue remodelling.

Key words: signalling proteins, apoptosis, cleft lip and palate, children.

Received: 19 06 2013

Accepted for publishing: 23 12 2013

¹Department of Morphology, Institute of Anatomy and Anthropology, Riga Stradins University, Latvia

²Institute of Stomatology, Riga Stradins University, Latvia

Liene Smane¹ – PhD student, lecturer

Mara Pilmane¹ – PhD., Dr. habil. med, prof.

Ilze Akota² – D.D.S., MSc (Oslo), PhD., prof.

Address correspondence to Liene Smane, Dzirciema street 16, Riga, LV-1007, Latvia.

E-mail address: Liene.Smane@rsu.lv



	Baltic Dental and Maxillofacial Journal

December, 2013, Vol. 15, No. 4 CONTENTS SCIENTIFIC ARTICLES Expression of growth factors and growth factor receptors in human cleft-affected tissue Benita Krivicka, Mara Pilmane, Ilze Akota 115-118 Radiographic assessment of findings in the maxillary sinus using cone-beam computed tomography Ilze Dobele, Ligija Kise, Peteris Apse, Gints Kragis, Andris Bigestans 119-122 Influence of restorative techniques on fracture load of endodontically treated premolars Ana Amélia Bianchi e Silva, Paula Cristine Ghiggi, Eduardo Gonçalves Mota, Gilberto Antonio Borges, Luiz Henrique Burnett Júnior, Ana Maria Spohr 123-128 Apoptosis and MMP-2, TIMP-2 expression in cleft lip and palate Liene Smane, Mara Pilmane, Ilze Akota 129-134 Toothache among dental patients attending a Nigerian secondary healthcare setting Clement Chinedu Azodo, Oritseweyemi Ololo 135-140 © 2014 Stomatologija		Stomatologija 2013; 15 (4): 129-134 455 KB Apoptosis and MMP-2, TIMP-2 expression in cleft lip and palate Liene Smane, Mara Pilmane, Ilze Akota Summary Aim of our study was complex detection of appearance and distribution of specific signalling proteins and apoptosis in facial tissue of children with complete bilateral cleft lip and palate (CBCLP). Materials and methods. Nineteen CBCLP patients and 11 unaffected subjects were involved in this study. All the tissue samples were proceeded for detection of matrix metalloproteinase-2 (MMP-2), tissue inhibitor of matrix metalloproteinase-2 (TIMP-2), and apoptosis. The intensity of immunostaining was graded semi-quantitatively. Results of the terminal deoxynucleotidyl transferase mediated dUTP nick-end labeling (TUNEL) method were obtained by counting apoptosis positive cells in five unintentionally chosen fields of vision. Groups were compared using the Mann-Whitney test. Results. TUNEL-positive oral epithelial cells were significantly increased in the control group when compared with the CBCLP group. Connective tissue cells have a statistically significant lower expression of TIMP-2 in the control group compared to the CBCLP group. Conclusions. TIMP-2 positive connective tissue cells increasingly found in oral mucosa lamina propria proves the decrease of local apoptosis in CLP patients. Prominent expression of MMP-2 in cleft affected soft tissue indicates a possible increase of tissue remodelling. Key words: signalling proteins, apoptosis, cleft lip and palate, children. Received: 19 06 2013 Accepted for publishing: 23 12 2013 ¹Department of Morphology, Institute of Anatomy and Anthropology, Riga Stradins University, Latvia ²Institute of Stomatology, Riga Stradins University, Latvia Liene Smane¹ – PhD student, lecturer Mara Pilmane¹ – PhD., Dr. habil. med, prof. Ilze Akota² – D.D.S., MSc (Oslo), PhD., prof. Address correspondence to Liene Smane, Dzirciema street 16, Riga, LV-1007, Latvia. E-mail address: Liene.Smane@rsu.lv