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Stomatologija 2013; 15 (4): 111-8 166 KB

Expression of growth factors and growth factor receptors in human cleft-affected tissue

Benita Krivicka, Mara Pilmane, Ilze Akota

Summary

Objective. To investigate cleft disordered tissue in children with cleft palate and cleft lip with or without alveolar clefting for detection of local tissue growth factors and growth factor receptors and compare findings.

Design. Morphological analysis of human tissue.

Patients. Three groups were studied: 14 patients with cleft palate at the age from eight months to 18 years and two months, 12 patients with cleft lip with or without alveolar clefting in the age from four months to 15 years and four months and 11 control patients.

Results. In general, cleft palate disordered tissue showed more prominent expression of BMP2/4 (z=3.574; p=0.0004) and TGFβ (z=2.127; p=0.033), while expression of TGFBR3 significantly higher was only in connective tissue (z=3.822; p=0.0001). Cleft lip affected tissue showed significantly pronounced expression of FGFR1 in general as well as separately in epithelium.

Conclusions. The marked and statistically significant expression of BMP 2/4 in cleft palate disordered soft tissue probably is delayed, but still proliferation and differentiation as well as tissue, especially, bone remodeling contributing signal. Cleft palate affected tissue show more prominent expression of TGFβ, still the weak regional expression of TGFβ type III receptors prove the disordered tissue growth and changed TGFβ signalling pathway in postnatal pathogenesis. In general, expression of TGFβ, BMP 2/4 and FGFR1 is significantly different, giving evidence to the involvement of these mentioned factors in the cleft severity morphopathogenesis.

Key words: TGFβ, TGFBR3, BMP2/4, bFGF, FGFR1.

Received: 12 07 2012

Accepted for publishing: 23 12 2013

¹Institute of Anatomy and Anthropology, Riga Stradins University, Riga, Latvia

²Department of Oral and Maxillofacial Surgery, Institute of Stomatology, Riga Stradins University, Riga, Latvia

Benita Krivicka¹ – PhD.

Mara Pilmane¹ – PhD., Dr. hab. med., prof.

Ilze Akota² – D.D.S., MSc (Oslo), PhD., prof.

Address correspondence to Dr. Benita Krivicka, Institute of Anatomy and Anthropology of Riga Stradins University, Dzirciema street 16, Riga, LV-1007, Latvia.

E-mail address: b.krivicka@inbox.lv



	Baltic Dental and Maxillofacial Journal

December, 2013, Vol. 15, No. 4 CONTENTS SCIENTIFIC ARTICLES Expression of growth factors and growth factor receptors in human cleft-affected tissue Benita Krivicka, Mara Pilmane, Ilze Akota 115-118 Radiographic assessment of findings in the maxillary sinus using cone-beam computed tomography Ilze Dobele, Ligija Kise, Peteris Apse, Gints Kragis, Andris Bigestans 119-122 Influence of restorative techniques on fracture load of endodontically treated premolars Ana Amélia Bianchi e Silva, Paula Cristine Ghiggi, Eduardo Gonçalves Mota, Gilberto Antonio Borges, Luiz Henrique Burnett Júnior, Ana Maria Spohr 123-128 Apoptosis and MMP-2, TIMP-2 expression in cleft lip and palate Liene Smane, Mara Pilmane, Ilze Akota 129-134 Toothache among dental patients attending a Nigerian secondary healthcare setting Clement Chinedu Azodo, Oritseweyemi Ololo 135-140 © 2014 Stomatologija		Stomatologija 2013; 15 (4): 111-8 166 KB Expression of growth factors and growth factor receptors in human cleft-affected tissue Benita Krivicka, Mara Pilmane, Ilze Akota Summary Objective. To investigate cleft disordered tissue in children with cleft palate and cleft lip with or without alveolar clefting for detection of local tissue growth factors and growth factor receptors and compare findings. Design. Morphological analysis of human tissue. Patients. Three groups were studied: 14 patients with cleft palate at the age from eight months to 18 years and two months, 12 patients with cleft lip with or without alveolar clefting in the age from four months to 15 years and four months and 11 control patients. Results. In general, cleft palate disordered tissue showed more prominent expression of BMP2/4 (z=3.574; p=0.0004) and TGFβ (z=2.127; p=0.033), while expression of TGFBR3 significantly higher was only in connective tissue (z=3.822; p=0.0001). Cleft lip affected tissue showed significantly pronounced expression of FGFR1 in general as well as separately in epithelium. Conclusions. The marked and statistically significant expression of BMP 2/4 in cleft palate disordered soft tissue probably is delayed, but still proliferation and differentiation as well as tissue, especially, bone remodeling contributing signal. Cleft palate affected tissue show more prominent expression of TGFβ, still the weak regional expression of TGFβ type III receptors prove the disordered tissue growth and changed TGFβ signalling pathway in postnatal pathogenesis. In general, expression of TGFβ, BMP 2/4 and FGFR1 is significantly different, giving evidence to the involvement of these mentioned factors in the cleft severity morphopathogenesis. Key words: TGFβ, TGFBR3, BMP2/4, bFGF, FGFR1. Received: 12 07 2012 Accepted for publishing: 23 12 2013 ¹Institute of Anatomy and Anthropology, Riga Stradins University, Riga, Latvia ²Department of Oral and Maxillofacial Surgery, Institute of Stomatology, Riga Stradins University, Riga, Latvia Benita Krivicka¹ – PhD. Mara Pilmane¹ – PhD., Dr. hab. med., prof. Ilze Akota² – D.D.S., MSc (Oslo), PhD., prof. Address correspondence to Dr. Benita Krivicka, Institute of Anatomy and Anthropology of Riga Stradins University, Dzirciema street 16, Riga, LV-1007, Latvia. E-mail address: b.krivicka@inbox.lv