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Stomatologija 2011; 13 (2): 55-61 228 KB

Comparative analysis of CRT Buffer, GC Saliva Check Buffer tests and laboratory titration to evaluate saliva buffering capacity

Ilze Maldupa, Anda Brinkmane, Anna Mihailova

Summary

Objective. The purpose of this study is to evaluate the ability of two commercial strip tests and laboratory titration to detect saliva buffer capacity.

Materials and methods. Sixty-four patients were examined. Stimulated saliva was collected and buffer capacity was determined with two different chair-side strip tests in addition to immediate transportation to the laboratory to check the buffering ability by titrating with 0.005 M HCl and measuring pH by digital pH/Ion meter, used as a gold standart. The correlation were analyzed using the Spearman Rank Correlation Test, Cohen’s Kappa coefficient and Pearson’s Correlation test, p < 0.01. Sensitivity and specificity were used to measure precision of these tests.

Results. The response rate was 80%. High buffer capacity was found in 23.4% of cases, medium in 62.5%, and low in 14.1%. The Spearman Rank Correlation coefficient between the titration method and CRT Buffer test was 0.685 and the GC Saliva Check Buffer was 0.837. The Kappa coefficient for the CRT Buffer test was 0.508, while the coefficient for the GC Saliva Check Buffer was 0.752. The Pearson Correlation for the GC Saliva Check was 0.675. The difference is found in the buffer capacity at initial pH and at pH value 3.
Conclusions. Both colorimetric tests correlate with the acid titration method in laboratory and are usable for saliva buffer capacity detection in dental offices. Buffer capacity detected in laboratory at different pH values can provide more information regarding caries risk.

Key words: saliva, caries risk, buffer capacity.

Received: 30 10 2010

Accepted for publishing: 24 06 2011

*Department of Therapeutic Dentistry, Institute of Stomatology, Riga Stradins University, Latvia

Ilze Maldupa* – PhD student

Anda Brinkmane* – D.D.S., PhD., assoc. prof.

Anna Mihailova* – postgraduate student

Address correspondence to Dr. Ilze Maldupa, T. Grothusa str. 2, Jelgava, LV- 3002.
E-mail address: ilze.maldupa@rsu.lv



	Baltic Dental and Maxillofacial Journal

June, 2011, Vol. 13, No. 2 CONTENTS SCIENTIFIC ARTICLES Proliferative potential in benign mixed salivary gland tumors and its value in primary and recurrent neoplasms Anna Kazanceva, Valerie Groma, Liene Smane, Egils Kornevs, Uldis Teibe 35-41 Relationship between radiographic changes in the temporomandibular joint and bone mineral density: A population based study Oksana Jagur, Mart Kull, Edvitar Leibur, Riina Kallikorm, Dagmar Loorits, Margus Lember, Ülle Voog – Oras 42-48 Prognostic factors and epidemiological characteristics of cutaneous and mucosal head and neck melanoma Anna Berzina, Kristine Azarjana, Ingrida Cema, Dace Pjanova, Alexander Rivosh 49-54 Comparative analysis of CRT Buffer, GC Saliva Check Buffer tests and laboratory titration to evaluate saliva buffering capacity Ilze Maldupa, Anda Brinkmane, Anna Mihailova 55-61 The relation between oral hygiene skills and the prevalence of dental caries among 4 - 6-yearoldchildren Jaunė Razmienė, Giedrius Vanagas, Eglė Bendoraitienė, Aurelija Vyšniauskaitė 62-67 Evaluation of oral health status of retirement-age population in Latvia Aldis Vidzis, Ingrida Cema, Ingrida Krasta, Anda Brinkmane, Imants Kalnins 68-72 © 2011 Stomatologija		Stomatologija 2011; 13 (2): 55-61 228 KB Comparative analysis of CRT Buffer, GC Saliva Check Buffer tests and laboratory titration to evaluate saliva buffering capacity Ilze Maldupa, Anda Brinkmane, Anna Mihailova Summary Objective. The purpose of this study is to evaluate the ability of two commercial strip tests and laboratory titration to detect saliva buffer capacity. Materials and methods. Sixty-four patients were examined. Stimulated saliva was collected and buffer capacity was determined with two different chair-side strip tests in addition to immediate transportation to the laboratory to check the buffering ability by titrating with 0.005 M HCl and measuring pH by digital pH/Ion meter, used as a gold standart. The correlation were analyzed using the Spearman Rank Correlation Test, Cohen’s Kappa coefficient and Pearson’s Correlation test, p < 0.01. Sensitivity and specificity were used to measure precision of these tests. Results. The response rate was 80%. High buffer capacity was found in 23.4% of cases, medium in 62.5%, and low in 14.1%. The Spearman Rank Correlation coefficient between the titration method and CRT Buffer test was 0.685 and the GC Saliva Check Buffer was 0.837. The Kappa coefficient for the CRT Buffer test was 0.508, while the coefficient for the GC Saliva Check Buffer was 0.752. The Pearson Correlation for the GC Saliva Check was 0.675. The difference is found in the buffer capacity at initial pH and at pH value 3. Conclusions. Both colorimetric tests correlate with the acid titration method in laboratory and are usable for saliva buffer capacity detection in dental offices. Buffer capacity detected in laboratory at different pH values can provide more information regarding caries risk. Key words: saliva, caries risk, buffer capacity. Received: 30 10 2010 Accepted for publishing: 24 06 2011 Department of Therapeutic Dentistry, Institute of Stomatology, Riga Stradins University, Latvia Ilze Maldupa – PhD student Anda Brinkmane* – D.D.S., PhD., assoc. prof. Anna Mihailova* – postgraduate student Address correspondence to Dr. Ilze Maldupa, T. Grothusa str. 2, Jelgava, LV- 3002. E-mail address: ilze.maldupa@rsu.lv