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Stomatologija 2017; 19 (4): 103-8 339 KB

Expression of gene proteins, interleukins and β-defensin in cleft-affected tissue

Iveta Jankovska¹, Mara Pilmane², Ilze Akota¹

Summary

Objectives. The aim of the study was to investigate cleft – affected tissues in children with cleft lip and palate in order to detect appearance of β-defensin, interleukins (IL) and gene proteins.

Material and methods. The study group included 10 patients with cleft lip and palate. During nose surgery tissue samples from bone and cartilage have been taken, and stained with immunohistochemistry for β-defensin 2, IL4, IL6, IL7, IL8, IL10 and MSX1, RYK, PAX9, IRF6 gene proteins.

Results. Results showed prominent expression of IL10 (mean value 47.28±4.26 in visual field) followed by IL7 (35.62±11.18) in cartilage of patients, but slightly less pronounced expression of IL8 (30.14±8.74), IL6 (22.52±10.88) and IL4 (14.81±6.94). The expression of β-defensin 2 was prominent (34.52±11.79) and similar to expression of IL7 in tissue samples of cartilage. MSX1, PAX9, RYK and IRF6 (17.67±5.94; 16.14±5.52; 16.57±5.22 and 11.86±4.21) in cartilage was less pronounced than interleukins and β-defensin 2. MSX1 (12.44±3.34), PAX9 (6.89±2.14), RYK (11.0±5.92) and IRF6 (9.1±4.76) gene proteins expression in bone showed mostly rare occurrence of positive structures.

Conclusions. Significant expression of IL7 and β-defensin 2 and IL10 in cartilage proves the prominent immune response in cleft affected hard tissue. Mostly indistinct MSX1, PAX9, RYK and IRF6 gene proteins expression in bone might be an indicator of not complete cellular differentiation, proliferation and migration events in cleft disordered hard tissue.

Key words: cleft lip and palate, immunohistochemistry.

Received: 08 05 2016

Accepted for publishing: 27 12 2017

¹Department of Orthodontics, Institute of Stomatology, Riga Stradins University, Riga, Latvia

²Department of Morphology, Institute of Anatomy and Anthropology, Riga Stradins University, Riga, Latvia

³Department of Oral and Maxillofacial Surgery, Institute of Stomatology, Rigas Stradins University, Riga,Latvia

Address correspondence to Dr. Iveta Jankovska, Department of Orthodontics, Institute of Stomatology, Riga Stradins University, 20 Dzirciema Street, Riga, Latvia.

E-mail address: iveta.j@hotmail.com



	Baltic Dental and Maxillofacial Journal

December, 2017, Vol. 19, No. 4 CONTENTS SCIENTIFIC ARTICLES Expression of gene proteins, interleukins and β-defensin in cleft-affected tissue Iveta Jankovska, Mara Pilmane, Ilze Akota 103-108 REVIEWS Host responses to Candida albicans. A review Eglė Zdanavičienė, Jurgina Sakalauskienė, Alvydas Gleiznys, Darius Gleiznys, Juozas Žilinskas 109-123 Locator® attachment system for implant overdentures: a systematic review Artur Miguel Quaresma Pereira Miler, André Ricardo Maia Correia, José Mário de Castro Rocha, José Carlos Reis Campos, Maria Helena Guimarães Figueiral da Silva 124-129 CASE REPORT The relevance of dental surgeon on Gorlin-Goltz syndrome Guilherme dos Santos Trento, Luis Fernando de Oliveira Gorla, Cláudia Maria Navarro, Valfrido Antônio Pereira Filho 130-132 © 2018 Stomatologija		Stomatologija 2017; 19 (4): 103-8 339 KB Expression of gene proteins, interleukins and β-defensin in cleft-affected tissue Iveta Jankovska¹, Mara Pilmane², Ilze Akota¹ Summary Objectives. The aim of the study was to investigate cleft – affected tissues in children with cleft lip and palate in order to detect appearance of β-defensin, interleukins (IL) and gene proteins. Material and methods. The study group included 10 patients with cleft lip and palate. During nose surgery tissue samples from bone and cartilage have been taken, and stained with immunohistochemistry for β-defensin 2, IL4, IL6, IL7, IL8, IL10 and MSX1, RYK, PAX9, IRF6 gene proteins. Results. Results showed prominent expression of IL10 (mean value 47.28±4.26 in visual field) followed by IL7 (35.62±11.18) in cartilage of patients, but slightly less pronounced expression of IL8 (30.14±8.74), IL6 (22.52±10.88) and IL4 (14.81±6.94). The expression of β-defensin 2 was prominent (34.52±11.79) and similar to expression of IL7 in tissue samples of cartilage. MSX1, PAX9, RYK and IRF6 (17.67±5.94; 16.14±5.52; 16.57±5.22 and 11.86±4.21) in cartilage was less pronounced than interleukins and β-defensin 2. MSX1 (12.44±3.34), PAX9 (6.89±2.14), RYK (11.0±5.92) and IRF6 (9.1±4.76) gene proteins expression in bone showed mostly rare occurrence of positive structures. Conclusions. Significant expression of IL7 and β-defensin 2 and IL10 in cartilage proves the prominent immune response in cleft affected hard tissue. Mostly indistinct MSX1, PAX9, RYK and IRF6 gene proteins expression in bone might be an indicator of not complete cellular differentiation, proliferation and migration events in cleft disordered hard tissue. Key words: cleft lip and palate, immunohistochemistry. Received: 08 05 2016 Accepted for publishing: 27 12 2017 ¹Department of Orthodontics, Institute of Stomatology, Riga Stradins University, Riga, Latvia ²Department of Morphology, Institute of Anatomy and Anthropology, Riga Stradins University, Riga, Latvia ³Department of Oral and Maxillofacial Surgery, Institute of Stomatology, Rigas Stradins University, Riga,Latvia Address correspondence to Dr. Iveta Jankovska, Department of Orthodontics, Institute of Stomatology, Riga Stradins University, 20 Dzirciema Street, Riga, Latvia. E-mail address: iveta.j@hotmail.com