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Stomatologija 2009; 11 (4): 113-118 345 KB

Early Morphofunctional Response of Contact Tissue after Intraosal Implantation in Rabbit Jaw of Pure Synthetic Hydroxyapatite (HAp) Bioceramic Materials and HAp Saturated with Lidocaine

Ilze Salma, Mara Pilmane, Andrejs Skagers, Janis Vetra, Girts Salms, Liga Berzina-Cimdina, Rita Serzane

Summary

Purpose. The aim of this study was to evaluate early morphofunctional response of contact tissue to pure and saturated with lidocaine porous synthetic HAp ceramic implants inserted in holes of rabbit upper jaw.

Materials and Methods. Pure and saturated with lidocaine cylinders of porous HAp ceramics produced at Biomaterials R&D Laboratory of Riga Technical University were inserted into edentulous part of the upper jaw of 12 New Zealand male rabbits. After two weeks, blocks of soft tissue and bone were taken out. The sections done with EXACT Grunding system and from paraffin blocks were stained with haematoxylin/eosin and by use of biotin-streptavidin immunohistochemistry for transforming growth factor β1 (TGFβ1), fibroblast growth factor receptor 1 (FGFR1), interleukin 10 (IL10). Apoptosis was detected by TUNEL method.

Results. TGFβ1 was equally expressed in the bone around HAp implants saturated with lidocaine and without lidocaine, while control sections did not show any expression of TGFβ. In contacting bone tissue to pure HAp and HAp saturated with lidocaine as well in controls rich expression of FGFR1 was observed. In soft tissue of experimental samples also rich expression of FGFR1 was observed, although it was weak in control samples. IL10 had rich expression in all samples. The mean number of apoptotic cells was higher in samples with pure HAp, slightly decreased in samples HAp with lidocaine and significantly lower in controls.

Conclusions. The expression of TGF β1 confirms osteoinductive activity of HAp. Contact soft tissue to HAp showed rich FGFR1 expression, and weak in controls suggesting higher plasticity of connective tissue to implants in comparison with supportive tissue.

Slightly lower apoptosis in samples of HAp with lidocaine gives evidence of indistinct influence of saturated with lidocaine HAp implants on development of programmed cell death.

Key words: implantation of hydroxyapatite in bone, lidocaine, transforming growth factor β, fibroblast growth factor receptor, interleukin 10, apoptosis.

Received: 09 03 2009

Accepted for publishing: 28 12 2009

¹Department of Oral and Maxillofacial Surgery, Riga Stradins University

²Institute of Anatomy and Anthropology, Riga Stradins University

³Center for Development and Innovation of Biomaterials, Riga Technical University

Ilze Salma¹ – MD, Mg (Biomaterials and Biomechanics), PhD student

Mara Pilmane² – MD, Dr. hab. med, professor, Director of Institute of Anatomy and Anthropology

Andrejs Skagers¹ – MD, Dr. hab. med, professor, Head of Department of Oral and Maxillofacial Surgery

Janis Vetra² – MD, Dr. hab. med., professor

Girts Salms¹ – MD, DDS, MRCSEd, Mg (Biomaterials and Biomechanics), lecturer

Liga Berzina-Cimdina³ – DrIng, Asoc.professor, Head Center for Development and Innovations of Biomaterials

Rita Serzane³ – PhD student, Faculty of Material Science and Applied Chemistry

Address correspondence to Ilze Salma, Riga Stradins University, Department of Oral and Maxillofacial Surgery, Dzirciema str. 20, Riga LV 1007, Latvia.
E-mail: salma.ilze@gmail.com



	Baltic Dental and Maxillofacial Journal

December, 2009, Vol. 11, No. 4 CONTENTS SCIENTIFIC ARTICLES Effects of different repolishing techniques on colour change of provisional prosthetic materials Vygandas Rutkunas, Vaidotas Sabaliauskas 105 -112 Early Morphofunctional Response of Contact Tissue after Intraosal Implantation in Rabbit Jaw of Pure Synthetic Hydroxyapatite (HAp) Bioceramic Materials and HAp Saturated with Lidocaine Ilze Salma, Mara Pilmane, Andrejs Skagers, Janis Vetra, Girts Salms, Liga Berzina-Cimdina, Rita Serzane 113-118 Relationship between tooth size discrepancies and malocclusion Kristina Lopatiene, Aiste Dumbravaite 119-124 Osteopontin and Osteocalcin in Maxilla Tissue of Skeletal Class III Patients Iveta Jankovska, Mara Pilmane, Ilga Urtane 125-128 In vitro investigation of the integration depth of oral fluids and disinfectants into alginate impressions Rimas Surna, Jonas Junevicius, Evaldas Rutkauskas 129-134 © 2009 Stomatologija		Stomatologija 2009; 11 (4): 113-118 345 KB Early Morphofunctional Response of Contact Tissue after Intraosal Implantation in Rabbit Jaw of Pure Synthetic Hydroxyapatite (HAp) Bioceramic Materials and HAp Saturated with Lidocaine Ilze Salma, Mara Pilmane, Andrejs Skagers, Janis Vetra, Girts Salms, Liga Berzina-Cimdina, Rita Serzane Summary Purpose. The aim of this study was to evaluate early morphofunctional response of contact tissue to pure and saturated with lidocaine porous synthetic HAp ceramic implants inserted in holes of rabbit upper jaw. Materials and Methods. Pure and saturated with lidocaine cylinders of porous HAp ceramics produced at Biomaterials R&D Laboratory of Riga Technical University were inserted into edentulous part of the upper jaw of 12 New Zealand male rabbits. After two weeks, blocks of soft tissue and bone were taken out. The sections done with EXACT Grunding system and from paraffin blocks were stained with haematoxylin/eosin and by use of biotin-streptavidin immunohistochemistry for transforming growth factor β1 (TGFβ1), fibroblast growth factor receptor 1 (FGFR1), interleukin 10 (IL10). Apoptosis was detected by TUNEL method. Results. TGFβ1 was equally expressed in the bone around HAp implants saturated with lidocaine and without lidocaine, while control sections did not show any expression of TGFβ. In contacting bone tissue to pure HAp and HAp saturated with lidocaine as well in controls rich expression of FGFR1 was observed. In soft tissue of experimental samples also rich expression of FGFR1 was observed, although it was weak in control samples. IL10 had rich expression in all samples. The mean number of apoptotic cells was higher in samples with pure HAp, slightly decreased in samples HAp with lidocaine and significantly lower in controls. Conclusions. The expression of TGF β1 confirms osteoinductive activity of HAp. Contact soft tissue to HAp showed rich FGFR1 expression, and weak in controls suggesting higher plasticity of connective tissue to implants in comparison with supportive tissue. Slightly lower apoptosis in samples of HAp with lidocaine gives evidence of indistinct influence of saturated with lidocaine HAp implants on development of programmed cell death. Key words: implantation of hydroxyapatite in bone, lidocaine, transforming growth factor β, fibroblast growth factor receptor, interleukin 10, apoptosis. Received: 09 03 2009 Accepted for publishing: 28 12 2009 ¹Department of Oral and Maxillofacial Surgery, Riga Stradins University ²Institute of Anatomy and Anthropology, Riga Stradins University ³Center for Development and Innovation of Biomaterials, Riga Technical University Ilze Salma¹ – MD, Mg (Biomaterials and Biomechanics), PhD student Mara Pilmane² – MD, Dr. hab. med, professor, Director of Institute of Anatomy and Anthropology Andrejs Skagers¹ – MD, Dr. hab. med, professor, Head of Department of Oral and Maxillofacial Surgery Janis Vetra² – MD, Dr. hab. med., professor Girts Salms¹ – MD, DDS, MRCSEd, Mg (Biomaterials and Biomechanics), lecturer Liga Berzina-Cimdina³ – DrIng, Asoc.professor, Head Center for Development and Innovations of Biomaterials Rita Serzane³ – PhD student, Faculty of Material Science and Applied Chemistry Address correspondence to Ilze Salma, Riga Stradins University, Department of Oral and Maxillofacial Surgery, Dzirciema str. 20, Riga LV 1007, Latvia. E-mail: salma.ilze@gmail.com