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Baltic Dental and Maxillofacial Journal

December, 2007, Vol. 9, No. 4

CONTENTS

SCIENTIFIC ARTICLES

Effect of cyclic 3´, 5´ adenosin-monophosphate on osteogenesis embryonic bone cultivated in tissue culture: an autoradiographic study
Edvitar Leibur, Elbi Lepp
99-104

The reduction of nitroblue tetrazolium by total blood in periodontitis patients and the aged
Juozas Zilinskas, Jonas Zekonis, Gediminas Zekonis, Aiste Valantiejiene, Rima Periokaite
105-108

Validity and reproducibility of cephalometric measurements obtained from digital photographs of analogue headfilms
Simonas Grybauskas, Irena Balciuniene, Janis Vetra
114-120

Finite element analysis of stresses in the maxillary and mandibular dental arches and TMJ articular discs during clenching into maximum intercuspation, anterior and unilateral posterior occlusion
Gaivile Pileicikiene, Algimantas Surna, Rimantas Barauskas, Rimas Surna, Algidas Basevicius
121-128

Dynamics of Pregnant Women's Oral Health Status during Preventive Programme
Ingrida Vasiliauskiene, Simona Milciuviene, Egle Bendoraitiene, Julija Narbutaite, Egle Slabsinskiene, Vilija Andruskeviciene
129-136

Stomatologija 2007; 9 (4): 99-104 1128 KB

Effect of cyclic 3´, 5´ adenosin-monophosphate on osteogenesis embryonic bone cultivated in tissue culture: An autoradiographic study

Edvitar Leibur, Elbi Lepp

Summary

The aim of this study was to evaluate the effect of cyclic 3´,5´adenosin-monophosphate (cAMP) on DNA synthesis of embryonic alveolar bone in tissue culture. Material and methods. Bone fragments were cultured in the medium composed of 80% medium 199, 15% horse serum, 4 mg/ml glucose, 100 mg/ml penicillin using the grid method.The explants were cultured up to 12 days. In the second series, the effect of cAMP in a concentration of 10^-6 M on bone during 12 days was studied. DNA synthesis was determined by calculating mitotic labelling indices for ³H-thymidine incorporation into cells within cultured explants at 3 to 12 days. The medium was supplemented with 1 microCurie/ml ³H-thymidine for 4 hours and processed for autoradiography. The mitotic labelling index was determined in the histological sections. All values wer presented as mean±standard deviation. Statistical significance was defined by p-values of 0,05 or less. Results. Morphological and statistical analysis showed that there were differences in mitotic incidence (MI) and silver grain densities over osteoblasts in control cultures and with cAMP. The mean value of MI was 4,627±1,001 in control and 7,706±1,188 in the cultures where cAMP was added (p<0,05). Conclusion. Thus cAMP inhibited bone resorption and stimulated new bone formation in tissue culture. This study provides a novel concept that may help to identify new therapeutic targets.

Key words: autoradiography, cAMP, osteogenesis, tissue culture.

Received: 04 10 2007

Accepted for publishing: 21 12 2007

¹Department of Stomatology, Department of Internal Medicine, Tartu University, Estonia
²Department of Morphology, Estonian University of Life Sciences, Tartu, Estonia

Edvitar Leibur¹ – MD, PhD, Dr.med.Sc., Dr.h.c. senior researcher, Prof. emeritus
Elbi Lepp² – Vet.D, PhD, docent

Address correspondence to Dr. Edvitar Leibur, Department of Stomatology, Tartu University Hospital, 8 Puusepa str., Tartu 51014, Estonia.
E-mail: Edvitar.Leibur@kliinikum.ee



	Baltic Dental and Maxillofacial Journal

December, 2007, Vol. 9, No. 4 CONTENTS SCIENTIFIC ARTICLES Effect of cyclic 3´, 5´ adenosin-monophosphate on osteogenesis embryonic bone cultivated in tissue culture: an autoradiographic study Edvitar Leibur, Elbi Lepp 99-104 The reduction of nitroblue tetrazolium by total blood in periodontitis patients and the aged Juozas Zilinskas, Jonas Zekonis, Gediminas Zekonis, Aiste Valantiejiene, Rima Periokaite 105-108 Factors related to job satisfaction among Lithuanian dentists Alina Puriene, Jadvyga Petrauskiene, Vilija Janulyte, Irena Balciuniene 109-113 Validity and reproducibility of cephalometric measurements obtained from digital photographs of analogue headfilms Simonas Grybauskas, Irena Balciuniene, Janis Vetra 114-120 Finite element analysis of stresses in the maxillary and mandibular dental arches and TMJ articular discs during clenching into maximum intercuspation, anterior and unilateral posterior occlusion Gaivile Pileicikiene, Algimantas Surna, Rimantas Barauskas, Rimas Surna, Algidas Basevicius 121-128 Dynamics of Pregnant Women's Oral Health Status during Preventive Programme Ingrida Vasiliauskiene, Simona Milciuviene, Egle Bendoraitiene, Julija Narbutaite, Egle Slabsinskiene, Vilija Andruskeviciene 129-136 © 2007 Stomatologija		Stomatologija 2007; 9 (4): 99-104 1128 KB Effect of cyclic 3´, 5´ adenosin-monophosphate on osteogenesis embryonic bone cultivated in tissue culture: An autoradiographic study Edvitar Leibur, Elbi Lepp Summary The aim of this study was to evaluate the effect of cyclic 3´,5´adenosin-monophosphate (cAMP) on DNA synthesis of embryonic alveolar bone in tissue culture. Material and methods. Bone fragments were cultured in the medium composed of 80% medium 199, 15% horse serum, 4 mg/ml glucose, 100 mg/ml penicillin using the grid method.The explants were cultured up to 12 days. In the second series, the effect of cAMP in a concentration of 10^-6 M on bone during 12 days was studied. DNA synthesis was determined by calculating mitotic labelling indices for ³H-thymidine incorporation into cells within cultured explants at 3 to 12 days. The medium was supplemented with 1 microCurie/ml ³H-thymidine for 4 hours and processed for autoradiography. The mitotic labelling index was determined in the histological sections. All values wer presented as mean±standard deviation. Statistical significance was defined by p-values of 0,05 or less. Results. Morphological and statistical analysis showed that there were differences in mitotic incidence (MI) and silver grain densities over osteoblasts in control cultures and with cAMP. The mean value of MI was 4,627±1,001 in control and 7,706±1,188 in the cultures where cAMP was added (p<0,05). Conclusion. Thus cAMP inhibited bone resorption and stimulated new bone formation in tissue culture. This study provides a novel concept that may help to identify new therapeutic targets. Key words: autoradiography, cAMP, osteogenesis, tissue culture. Received: 04 10 2007 Accepted for publishing: 21 12 2007 ¹Department of Stomatology, Department of Internal Medicine, Tartu University, Estonia ²Department of Morphology, Estonian University of Life Sciences, Tartu, Estonia Edvitar Leibur¹ – MD, PhD, Dr.med.Sc., Dr.h.c. senior researcher, Prof. emeritus Elbi Lepp² – Vet.D, PhD, docent Address correspondence to Dr. Edvitar Leibur, Department of Stomatology, Tartu University Hospital, 8 Puusepa str., Tartu 51014, Estonia. E-mail: Edvitar.Leibur@kliinikum.ee