www.sbdmj.com - Stomatologija - Baltic Dental and Maxillofacial Journal

Summary

Growth factors and growth stimulating genes are main signaling molecules for growth and development in ante– and postnatal period involved in cellular proliferation, differentiation and morphogenesis of tissues and organs during embryogenesis, postnatal growth and adulthood. The aim of this study was to evaluate TGF-β (transforming growth factor-β), BMP2/4 (bone morphogenetic protein 2/4), FGFR1 (fibroblast growth factor receptor one), barx1 gene and apoptosis from tissue samples of oro-maxillo-facial region in skeletal class III patients to reveal possible morphopathogenesis of severe skeletal anomalies. The study group included 9 patients with skeletal class III malocclusion. During orthognatic surgery tissue samples from tuber maxillae, ramus mandibulae anterior and posterior part, as well as gingiva from the lower jaw in region of second molar have been taken. Samples were stained with immunohistochemistry for TGF-β, BMP2/4, FGFR1, apoptosis and barx1 gene. We used also the routine histological staining with haematoxyline and eosine.

In tuber maxillae, ramus mandibulae anterior and posterior part staining for TGF-β was the most relevant. Also BMP2/4, FGFR1 and barx1 showed the highest mean number of positive cells in tuber maxillae. Barx1 was equally expressed in ramus mandibulae, but BMP2/4 and FGFR1 mainly stained its posterior part cells. Apoptosis mostly affected ramus mandibulae anterior part.

Conclusions. We suggest about more active stimulation of bone growth in tuber maxilla whereas ramus mandibulae. Apoptosis mainly affects ramus mandibulae anterior part that possibly connects to the lower expression of growth stimulating factors and may indicate lower bone remodelation ability.

Key words: growth factors, apoptosis, orthognathic surgery, immuno-histochemistry

Received: 17 04 2007

Accepted for publishing: 10 07 2007

¹Department of Orthodontics, Institute of Stomatology, Riga Stradins University, Latvia

²Institute of Anatomy and Anthropology, Riga Stradins University, Latvia

³Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Riga Stradins University, Latvia

Iveta Jankovska¹ – D.D.S.

Mara Pilmane² – Dr. med., Dr. hab. med., Corresponding member of Latvian Academy of Science, Director of Institute of Anatomy and Anthropology

Ilga Urtane¹ – D.D.S., Dr. med., Professor and Head of Department of Ortho-dontics, Director of Institute of Stomatology

Andris Bigestans³ – M.D., D.D.S., lecturer

Girts Salms³ – M.D., D.D.S., lecturer

Gunars Lauskis³ – M.D., D.D.S., lecturer

Address correspondence to Dr. Iveta Jankovska, Department of Orthodontics, Institute of Stomatology, Riga Stradins University, 20 Dzirciema Street, Riga, Latvia, LV 1007.
E-mail: iveta.j@one.lv



	Baltic Dental and Maxillofacial Journal

June, 2007, Vol. 9, No. 2 CONTENTS REVIEWS The diagnostic process for temporomandibular disorders 35-39 SCIENTIFIC ARTICLES Growth factors, apoptotic cells and barx1 gene in bone and soft tissue of skeletal class III patients 40-46 Reliability of ARCUSdigma (KaVo®) in diagnosing temporomandibular joint pathology 47-55 Influence of the mechanical properties of composites for indirect dental restorations on pattern failure 56-60 CLINICAL CASE REPORTS Sarcoidosis (Heerfordt syndrome): A case report 61-64 © 2007 Stomatologija		Stomatologija 2007; 9 (2): 40-46 655 KB *Growth factors, apoptotic cells and barx1* gene in bone and soft tissue of skeletal class III patients Iveta Jankovska, Mara Pilmane, Ilga Urtane, Andris Bigestans, Girts Salms, Gunars Lauskis Summary** Growth factors and growth stimulating genes are main signaling molecules for growth and development in ante– and postnatal period involved in cellular proliferation, differentiation and morphogenesis of tissues and organs during embryogenesis, postnatal growth and adulthood. The aim of this study was to evaluate TGF-β (transforming growth factor-β), BMP2/4 (bone morphogenetic protein 2/4), FGFR1 (fibroblast growth factor receptor one), barx1 gene and apoptosis from tissue samples of oro-maxillo-facial region in skeletal class III patients to reveal possible morphopathogenesis of severe skeletal anomalies. The study group included 9 patients with skeletal class III malocclusion. During orthognatic surgery tissue samples from tuber maxillae, ramus mandibulae anterior and posterior part, as well as gingiva from the lower jaw in region of second molar have been taken. Samples were stained with immunohistochemistry for TGF-β, BMP2/4, FGFR1, apoptosis and barx1 gene. We used also the routine histological staining with haematoxyline and eosine. In tuber maxillae, ramus mandibulae anterior and posterior part staining for TGF-β was the most relevant. Also BMP2/4, FGFR1 and barx1 showed the highest mean number of positive cells in tuber maxillae. Barx1 was equally expressed in ramus mandibulae, but BMP2/4 and FGFR1 mainly stained its posterior part cells. Apoptosis mostly affected ramus mandibulae anterior part. Conclusions. We suggest about more active stimulation of bone growth in tuber maxilla whereas ramus mandibulae. Apoptosis mainly affects ramus mandibulae anterior part that possibly connects to the lower expression of growth stimulating factors and may indicate lower bone remodelation ability. Key words: growth factors, apoptosis, orthognathic surgery, immuno-histochemistry Received: 17 04 2007 Accepted for publishing: 10 07 2007 ¹Department of Orthodontics, Institute of Stomatology, Riga Stradins University, Latvia ²Institute of Anatomy and Anthropology, Riga Stradins University, Latvia ³Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Riga Stradins University, Latvia Iveta Jankovska¹ – D.D.S. Mara Pilmane² – Dr. med., Dr. hab. med., Corresponding member of Latvian Academy of Science, Director of Institute of Anatomy and Anthropology Ilga Urtane¹ – D.D.S., Dr. med., Professor and Head of Department of Ortho-dontics, Director of Institute of Stomatology Andris Bigestans³ – M.D., D.D.S., lecturer Girts Salms³ – M.D., D.D.S., lecturer Gunars Lauskis³ – M.D., D.D.S., lecturer Address correspondence to Dr. Iveta Jankovska, Department of Orthodontics, Institute of Stomatology, Riga Stradins University, 20 Dzirciema Street, Riga, Latvia, LV 1007. E-mail: iveta.j@one.lv