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Summary

Aim of study was to examinate periodontal status among 20 44 year old patients and to study the secretory function of peripheral venous blood neutrophilic leukocytes (NL) exposed to various antigens and alkaline phosphatase (AP) activity in gingival crevicular fluid (GCF) in patients suffering from gingivitis and periodontitis. Clinicaly were determinted Russell's periodontal index (PI). Secretory function of NL affected by opsonized zymosane, non-opsonized E. coli was examinated in 77 patients with gingivitis and periodontitis, and in 35 donors, free of internal diseases, by means of β-glucuronidase (β-GD), lysozyme (LZ). NL secreted higher levels of β-GD in incubation medium in patients with periodontitis (p≤0.001) subject to degree of periodontal lesion. NL affected by various antigens secreted higher levels of LZ into non-cellular matrix in patients with gingivitis and periodontitis comparing to control environment in analogous groups. Data obtained from this study suggest that in patients with periodontitis response of NL to bacterial stimuli is specific and subject to the degree of periodontal lesion. Our study showed a significant difference of AP activity in GCF subject to pocket depth and degree of periodontal lesion. Once NL are exposed to corpuscules prone to phagocytosis, an increase in secretion of β-GD and LZ can be explained by overall increase in secretion of NL lysosomic enzymes, thus disclosing the mechanism of inflammatory periodontal tissue damage.

Key words: periodontal diseases, alkaline phosphatase, β-glucuronidase, lysozyme.

Received: 29 03 2005

Accepted for publishing: 20 08 2005

¹Clinic of Prosthetic Denstistry, Kaunas Medical University, Lithuania

²Department of Dental and Maxillar Orthopedics, Kaunas University of Medicine, Lithuania

Jurgina Sakalauskienė¹ - D.D.S., PhD, assoc. prof.

Algimantas Šurna² - D.D.S., PhD, assoc. prof.

Eglė Ivanauskienė¹ - D.D.S., assist. prof.

Gediminas Žekonis¹ - D.D.S., PhD, lecturer.

Alvydas Gleiznys² - D.D.S., PhD, assoc. prof.

Address correspondence to Dr. Jurgina Sakalauskiene, Clinic of Prosthetic Dentistry, Sukilėlių 51, Kaunas, Lithuania.



	Baltic Dental and Maxillofacial Journal

September, 2005, Vol. 7, No. 3 CONTENTS SCIENTIFIC ARTICLES Jaw tracking devices - historical review of methods development. Part I 67 - 71 Jaw tracking devices - historical review of methods development. Part II 72 - 76 The masticatory system - an overview 77 - 80 Oral parafunctions and positive clinical examination findings 81 - 83 Testing of the questionnaire on dental care satisfaction in a sample of adult patients visiting dental clinics at Faculty of Odontology, Kaunas University of Medicine. A Pilot study 84 - 89 Secretory function of neutrophilic leukocytes of the patients with periodontal diseases 90 - 94 5 year experience with lower lip cancer 95 - 98 © 2005 Stomatologija		Stomatologija 2005; 7 (3): 90-4 207 KB Secretory function of neutrophilic leukocytes of the patients with periodontal diseases Jurgina Sakalauskienė, Algimantas Šurna, Eglė Ivanauskienė, Gediminas Žekonis, Alvydas Gleiznys Summary Aim of study was to examinate periodontal status among 20 44 year old patients and to study the secretory function of peripheral venous blood neutrophilic leukocytes (NL) exposed to various antigens and alkaline phosphatase (AP) activity in gingival crevicular fluid (GCF) in patients suffering from gingivitis and periodontitis. Clinicaly were determinted Russell's periodontal index (PI). Secretory function of NL affected by opsonized zymosane, non-opsonized E. coli was examinated in 77 patients with gingivitis and periodontitis, and in 35 donors, free of internal diseases, by means of β-glucuronidase (β-GD), lysozyme (LZ). NL secreted higher levels of β-GD in incubation medium in patients with periodontitis (p≤0.001) subject to degree of periodontal lesion. NL affected by various antigens secreted higher levels of LZ into non-cellular matrix in patients with gingivitis and periodontitis comparing to control environment in analogous groups. Data obtained from this study suggest that in patients with periodontitis response of NL to bacterial stimuli is specific and subject to the degree of periodontal lesion. Our study showed a significant difference of AP activity in GCF subject to pocket depth and degree of periodontal lesion. Once NL are exposed to corpuscules prone to phagocytosis, an increase in secretion of β-GD and LZ can be explained by overall increase in secretion of NL lysosomic enzymes, thus disclosing the mechanism of inflammatory periodontal tissue damage. Key words: periodontal diseases, alkaline phosphatase, β-glucuronidase, lysozyme. Received: 29 03 2005 Accepted for publishing: 20 08 2005 ¹Clinic of Prosthetic Denstistry, Kaunas Medical University, Lithuania ²Department of Dental and Maxillar Orthopedics, Kaunas University of Medicine, Lithuania Jurgina Sakalauskienė¹ - D.D.S., PhD, assoc. prof. Algimantas Šurna² - D.D.S., PhD, assoc. prof. Eglė Ivanauskienė¹ - D.D.S., assist. prof. Gediminas Žekonis¹ - D.D.S., PhD, lecturer. Alvydas Gleiznys² - D.D.S., PhD, assoc. prof. Address correspondence to Dr. Jurgina Sakalauskiene, Clinic of Prosthetic Dentistry, Sukilėlių 51, Kaunas, Lithuania.