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Stomatologija 2003; 5 (1): 9-12 184 KB

Electrophoresis of Whole-cell Soluble Proteins of Enterococcus faecalis and Yeast Isolated in the Root Canals of Previously Root-filled Teeth

Vytaute Peciuliene, Irena Balciuniene, Markus Haapasalo

Summary

Microbiological samples were taken from the root canals of 40 previously root filled teeth with apical periodontitis requiring retreatment with special emphasis on the occurrence of E. faecalis and C. albicans. Microbes were isolated from 33 out of the 40 teeth. Preliminary characterisation of microbial species found in the root canals was based on aerotolerance, gram staining and production of catalase and indole. Identification to species level was based on motility test and carbohydrate fermentation patterns. The detection of glycosidase and aminopeptidase enzymes was used for identification of facultative gram negative enteric rods. Streptococcal isolates growing on bile-esculin agar plates were further identified to species level by a commercial test panel. Identification of Yeasts was based on cellular appearance in gram stained smears and on production of glycosidase enzymes. Results obtained from the primary tests may give inconsistently expressed phenotypic traits.

In order to confirm the results of primary identification tests, electrophoresis of whole-cell soluble proteins as valuable method for rapid and precise characterisation of oral bacteria was used. This test have showed that the protein patterns of reference strain of E. faecalis and C. albicans exhibited identical or similar characteristics with protein patterns of clinical strains of E. faecalis and C. albicans isolated from the root canals and it confirmed primary tests results.

E. faecalis was found in 21 out of the 33 culture positive root canals. C. albicans was found in 6 out of 33 culture positive root canals.

Key words: E. faecalis, C. albicans, electrophoresis of whole-cell soluble proteins, phenotypic traits

Address correspondence to Dr.Vytaute Peciuliene: 115 Zalgirio str., Vilnius, Lithuania. E-mail: vytautep@delfi.lt

Received: 20 02 2003

Accepted for publishing: 25 03 2003



	Baltic Dental and Maxillofacial Journal

2003, Vol. 5, No. 1 CONTENTS Analysis of Experimental and Clinical Research of Destruction of Peripheral Branches of the Trigeminal Nerve Electrophoresis of Whole-cell Soluble Proteins of Enterococcus faecalis and Yeast Isolated in the Root Canals of Previously Root-filled Teeth Pharyngeal Airway Sagittal Dimension in Patients with Class III Skeletal Dentofacial Deformity Before and After Bimaxillary Surgery Late Results of the Secondary Alveolar Bone Grafting in Complete Unilateral Cleft Lip and Palate Patients Prediction of Malocclusion Development Based on the Evaluation of the Ethiologic Factors Analysis of Tooth Size Discrepancy (Bolton Index) among Patients of Orthodontic Clinic at Kaunas Medical University Oral Hygiene Habits and Complaints of Gum Bleeding Among Schoolchildren in Lithuania © 2003, Stomatologija		Stomatologija 2003; 5 (1): 9-12 184 KB Electrophoresis of Whole-cell Soluble Proteins of Enterococcus faecalis and Yeast Isolated in the Root Canals of Previously Root-filled Teeth Vytaute Peciuliene, Irena Balciuniene, Markus Haapasalo Summary Microbiological samples were taken from the root canals of 40 previously root filled teeth with apical periodontitis requiring retreatment with special emphasis on the occurrence of E. faecalis and C. albicans. Microbes were isolated from 33 out of the 40 teeth. Preliminary characterisation of microbial species found in the root canals was based on aerotolerance, gram staining and production of catalase and indole. Identification to species level was based on motility test and carbohydrate fermentation patterns. The detection of glycosidase and aminopeptidase enzymes was used for identification of facultative gram negative enteric rods. Streptococcal isolates growing on bile-esculin agar plates were further identified to species level by a commercial test panel. Identification of Yeasts was based on cellular appearance in gram stained smears and on production of glycosidase enzymes. Results obtained from the primary tests may give inconsistently expressed phenotypic traits. In order to confirm the results of primary identification tests, electrophoresis of whole-cell soluble proteins as valuable method for rapid and precise characterisation of oral bacteria was used. This test have showed that the protein patterns of reference strain of E. faecalis and C. albicans exhibited identical or similar characteristics with protein patterns of clinical strains of E. faecalis and C. albicans isolated from the root canals and it confirmed primary tests results. E. faecalis was found in 21 out of the 33 culture positive root canals. C. albicans was found in 6 out of 33 culture positive root canals. Key words: E. faecalis, C. albicans, electrophoresis of whole-cell soluble proteins, phenotypic traits Address correspondence to Dr.Vytaute Peciuliene: 115 Zalgirio str., Vilnius, Lithuania. E-mail: vytautep@delfi.lt Received: 20 02 2003 Accepted for publishing: 25 03 2003