|Baltic Dental and Maxillofacial Journal|
September, 2011, Vol. 13, No. 3
CLINICAL CASE REPORTS
© 2011 Stomatologija
Stomatologija 2011; 13 (3): 75-80 297 KB
In vitro evaluation of cytotoxicity of permanent prosthetic materials
Vaidotas Sabaliauskas, Rima Juciute, Virginija Bukelskiene, Vygandas Rutkunas, Rita Trumpaite-Vanagiene, Alina Puriene
Objectives. To assess qualitative and quantitative cytotoxicity effect on permanent prosthetic materials to human gingival fibroblasts.
Methods. Human gingival tissues were collected (with informed consent) from patients undergoing periodontal surgical procedures and fibroblasts were cultured in vitro. Cell type was determined by performing proteomic analysis. Selected prosthetic materials including titanium, feldspathic ceramic, gold and chrome-cobalt alloy specimens (5×2 mm) were fabricated. The toxicity of prepared specimens was tested by exposing them to cell culture medium for 48, 72, 96 and 120 hours at 37°C under sterile conditions. Cell viability was estimated using MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay. The data concerning cell viability were statistically analyzed using two-way ANOVA test and Tukey multiple comparison test.
Results. Results obtained after 48 hours showed no toxic effect of titanium compared to control group. Cytotoxic effect was observed in gold alloy and feldspathic ceramic, however, it was not significant compared to control group. Chrome-cobalt alloy significantly reduced cell viability compared to control group (p≤0.001). Cytotoxicity diminished with increasing incubation time of specimens.
After 120 hours of incubation all tested materials, except chrome-cobalt alloy, had no cytotoxicity.
Conclusions. Titanium proved to be non-toxic. Gold alloy and feldspathic ceramic had short-term cytotoxic effect. Chrome-cobalt alloy had highest cytotoxic effect on fibroblast cells.
Key words: cytotoxicity, prosthetic materials, gold alloy, feldspathic ceramic, titanium, chrome-cobalt alloy, human gingival cells, fibroblasts.
Received: 03 12 2010
Accepted for publishing: 23 09 2011
1Institute of Odontology, Faculty of Medicine, Vilnius University, Lithuania
2Vilnius University Zalgirio clinics, Lithuania
3Department of Development Biology, Institute of Biochemistry, Lithuania
Vaidotas Sabaliauskas1 D.D.S.
Rima Juciute2 D.D.S.
Vygandas Rutkunas1, 2 D.D.S., PhD, assoc. prof.
Virginija Bukelskiene3 PhD
Rita Trumpaite-Vanagiene1, 2 D.D.S., assist. prof.
Alina Puriene1, 2 D.D.S., PhD, prof.
Address correspondence to Dr. Vaidotas Sabaliauskas, Institute of Odontology, Faculty of Medicine, Vilnius University, Zalgirio str. 115-219, Vilnius, Lithuania.
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