Baltic Dental and Maxillofacial Journal
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June, 2005, Vol. 7, No. 2

CONTENTS

SCIENTIFIC ARTICLES

Comparative analysis of the effectiveness of the mandibular angle fracture treatment methods
35 - 39

Chemomechanical caries removal for children
40 - 44

The microbiological status of patients with periodontitis in Southern Estonia after non-surgical periodontal therapy
45 - 47

The response of peripheral blood neutrophils unstimulated and stimulated by prodigiosan to smoking
48 - 51

Effectiveness evaluation of different suction systems
52 - 57

Dietary and oral hygiene habits in children with type I Diabetes Mellitus related to dental caries
58 - 62

© 2005 Stomatologija

Stomatologija 2005; 7 (2): 48-51 190 KB

The Response of Peripheral Blood Neutrophils Unstimulated and Stimulated by Prodigiosan to Smoking

Darius Zasimauskas, Gediminas Žekonis

Summary

Alterations of neutrophils function by tobacco products may play a central role in the pathogenesis of periodontal diseases and several smoking related systemic diseases. The aim of the study was to assess a response of peripheral blood neutrophils unstimulated and stimulated by prodigiosan to smoking.

Materials and methods. The study included 17 smoking men that addressed for treatment of various odontological problems to out patient department of Kaunas University Clinic. The subjects were 22-43 years of age and systemic diseases free. All subjects answered to questions about smoking habits. To assess the response of neutrofils (unstimulated and stimulated by prodigiosan) to smoking the chemiliuminescence and nitroblue tetrazolium test were used to measure their oxidative metabolism.

Results. After smoking both neutrophils unstimulated and stimulated with prodigiosan showed considerable extracellular chemiliuminescence responses, but the first one reached maximal value in 45 min, while the second one in 15 min after smoking. There was no total (intra and extracellular) chemiliuminescence response of neutrophils unstimulated and stimulated by prodigiosan to smoking. Neutrofils exposed to smoking showed a significant increase in their nitroblue tetrazolium reduction.

Conclusion. Both stimulated by prodigiosan and unstimulated peripheral blood neutrophils release reactive oxygen species extracellulary which may alter the pathogenic processes in periodontal and other systemic diseases.

Key words: neutrophils, smoking, prodigiosan, reactive oxygen species, chemiluminescence, nitroblue tetrazolium reduction test.

Received: 20 04 2005

Accepted for publishing: 20 06 2005


Darius Zasimauskas - D.D.S., assist. prof., Clinic of Prosthetic Denstistry, Kaunas Medical University, Lithuania.

Gediminas Zekonis - D.D.S., PhD, assoc. prof., Clinic of Prosthetic Denstistry, Kaunas Medical University, Lithuania.


Address correspondence to Gediminas Zekonis, Clinic of Prosthetic Dentistry, Hospital of Kaunas University of Medicine, Sukilelių 51, Kaunas, Lithuania.
E-mail: zekonisg@hotmail.com